Journal: Bioactive Materials

Article Title: Degradation-tunable coating with sustained silver release for spinal implants to prevent postoperative infections

doi: 10.1016/j.bioactmat.2026.02.035

Figure Lengend Snippet: Evaluation of the cytotoxicity and osteogenic properties of AgZ/GelSF coatings. (a) The cytotoxicity of extracts from coatings with different AgZ loadings on Ti discs was assessed using the CCK-8 assay after incubation for 24 h. The amount of GelSF applied was 10 mg per disc. (b) The cytotoxicity of extracts from coatings with varying AgZ/GelSF fill amounts on Ti-6Al-4V cages was assessed using the CCK-8 assay after incubation for 24 h. Based on the results from panel (a), the Ag loading was fixed at 0.25 mg per 10 mg GelSF. AgZ/GelSF-38, 55, and 82 represent coating fill amounts of 38, 55, and 82 mg AgZ/GelSF per 10 g of cage, respectively. (c) Fluorescence images showing the viability of cells cultured in extracts from coatings with different AgZ/GelSF loadings. (d) Confocal microscopy images showing the growth status of cells cultured in various extracts. Green fluorescence indicates FITC-labeled actin filaments, while blue fluorescence indicates DAPI-stained nuclei. (e) Migration of cells cultured in material extracts for 12 and 24 h, visualized by fluorescence imaging. (f) ALP activity after 7 days of culture in extracts. (g) ARS staining after 14 days of culture, with insets showing macroscopic views of the staining. Based on the results from panel (b), the concentration of extracts used in panels (c-g) was 50%. (h) Schematic of rabbit femoral condyle bone defect model: the AgZ/GelSF coating was applied to a Ti-6Al-4V implant (Φ 6 mm × 9 mm) at a ratio of 55 mg per 10 g of implant, based on previous results. Osteogenic effects on the implant surface were evaluated after 6 weeks. (i) 3D and 2D reconstructed Micro-CT images showing bone tissue surrounding the implant. (j) Quantification of bone formation based on Micro-CT analysis, expressed as the ratio of bone volume to tissue volume. (k) Histological analysis of bone formation: top-view images of hard tissue sections from the femur stained with methylene blue/acid fuchsin. Yellow arrows indicate new bone formation on the implant surface. All data are presented as mean ± standard deviation from three independent samples.

Article Snippet: Cell viability was assessed using a CCK-8 assay kit (HY-K0301, MedChemExpress, USA).

Techniques: CCK-8 Assay, Incubation, Fluorescence, Cell Culture, Confocal Microscopy, Labeling, Staining, Migration, Imaging, Activity Assay, Concentration Assay, Micro-CT, Standard Deviation

Journal: Poultry Science

Article Title: Brefeldin A inhibits duck plague virus replication by impairing virion envelopment

doi: 10.1016/j.psj.2026.106509

Figure Lengend Snippet: Brefeldin A inhibits DPV infection in DEF cells . (A-C) DEF cells were infected with DPV-CHv-GFP at 1 MOI for 1 h, then treated with different concentrations of Brefeldin A, DMSO was added as control. The viral fluorescence spots were photographed under fluorescence microscope at 24 hours post infection (hpi). The scale bar is 100 µm (A). The infected cells were collected and the copy number of the DPV gene was detected by Q-PCR (B), and the cell supernatant was collected to determine the virus titer TCID 50 (C). (D) DEF cells were treated with different concentrations of Brefeldin A for 48 h, and then the cell viability was determined using the CCK-8 cell viability assay kit.

Article Snippet: DEF cells were treated with BFA of different concentrations for 36 h, and the cell viability was determined using the cell counting CCK-8 kit (TargetMOI, c0005) according to the manufacturer's instructions.

Techniques: Infection, Control, Fluorescence, Microscopy, Virus, CCK-8 Assay, Viability Assay